T4 DNA Ligase with PEG, 5u/ul 1KU

Description

Introduction

T4 DNA Ligase with PEG is a premium-grade enzymatic solution engineered for the rapid ligation of DNA fragments. By incorporating polyethylene glycol (PEG) into the reaction buffer, this formulation significantly enhances the speed of ligation, making it an indispensable tool for molecular biology research and routine cloning procedures.

Key Features

– Optimized concentration of 5 units per microliter for precise reaction control.

– Formulated with PEG to accelerate ligation rates for both cohesive and blunt ends.

– Provides superior transformation efficiency in standard bacterial hosts.

– Stable formulation ensures consistent activity across repeated freeze-thaw cycles.

– Compatible with a wide range of downstream applications including NGS library prep.

– Fast reaction times ranging from 5 to 15 minutes at room temperature.

Benefits

This product solves the common bottleneck of slow ligation times in cloning workflows. By utilizing the molecular crowding effect of PEG, the ligase effectively brings DNA ends into proximity, drastically reducing incubation times from hours to minutes. Researchers can achieve higher colony counts and successful ligations even with challenging blunt-ended fragments, ultimately saving valuable time and reducing the cost of reagents per experiment.

Usage / How to Use

The T4 DNA Ligase is designed for standard ligation reactions. To use, simply combine your linearized vector and insert DNA in a microcentrifuge tube, add the provided buffer and the T4 DNA Ligase enzyme, and incubate at room temperature for 5 to 15 minutes. The reaction can then be used directly for chemical or electro-transformation into competent cells. Always keep the enzyme on ice during use to maintain peak activity.

Why Choose This Product

– Guaranteed high activity levels for reliable, repeatable results every time.

– Time-saving formulation reduces your total cloning cycle by several hours.

– Stringently quality-tested to ensure freedom from nucleases and inhibitors.

– Trusted by laboratories for robust performance in high-stakes gene editing projects.

– Economical pack size minimizes waste while maximizing throughput.