RTD100 Total RNA Mini Kit (Tissue) with DNase Set
Achieve superior RNA purity with the RTD100 Total RNA Mini Kit. Designed for high-yield extraction from diverse tissue samples, this kit features an integrated DNase set for DNA-free results. Perfect for sensitive downstream applications like RT-PCR and Northern blotting, it ensures consistent, reliable performance for your most critical laboratory research workflows.
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Description
Introduction
The RTD100 Total RNA Mini Kit provides a streamlined solution for the rapid isolation of high-quality total RNA from a wide variety of animal and plant tissue samples. This all-inclusive kit incorporates a robust buffer system and an optimized DNase treatment step to ensure that your final RNA preparation is free from genomic DNA contamination.
Key Features
– Optimized for high-yield RNA recovery from challenging tissue types
– Includes a dedicated DNase I set for efficient on-column DNA digestion
– Rapid spin-column format reduces processing time to under 30 minutes
– High purity ratios suitable for sensitive downstream enzymatic reactions
– Scalable protocol to handle varying tissue input amounts
– Stable reagents that ensure consistent experimental reproducibility
Benefits
The primary challenge in RNA research is maintaining sample integrity while effectively removing DNA. This kit eliminates the need for time-consuming extra steps by integrating the DNase treatment directly into the purification process. Researchers benefit from a significantly simplified workflow that saves time without compromising yield. The resulting high-purity RNA is ready for immediate use in demanding applications such as RT-qPCR, transcriptome analysis, and gene expression studies, ensuring your data is accurate and reliable.
Usage / How to Use
This kit is intended for professional laboratory use. Simply homogenize your tissue sample using an appropriate lysis buffer, then apply the lysate to the provided spin column. After washing away contaminants, add the DNase I solution directly to the column to digest residual DNA. After a final wash and short centrifugation, high-purity total RNA is eluted in nuclease-free water, ready for your research experiments.
Why Choose This Product
– Proven reliability in rigorous laboratory settings
– Cost-effective solution offering 100 preps per kit
– Comprehensive set includes all necessary buffers and enzymes
– Designed to minimize RNA degradation during isolation
– Excellent customer support and technical documentation provided
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