RIPA Buffer I 2X Concentrate pH 7.4 – BC4475

Description

Introduction

The BC4475 RIPA Buffer I is a high-quality 2X concentrate solution engineered for robust protein extraction from mammalian cells and tissues. Designed at an optimal pH of 7.4, this buffer provides the ideal environment for effective cell lysis while maintaining the integrity and stability of your target proteins.

Key Features

– Optimized 2X concentration for flexible experimental design

– Precisely balanced pH 7.4 for maximum protein recovery

– Compatible with most standard protein assays including BCA and Bradford

– Effectively solubilizes cytoplasmic, nuclear, and membrane proteins

– Minimizes protein degradation through high-purity raw materials

– Suitable for immunoprecipitation and Western Blotting workflows

Benefits

When working with complex biological samples, protein yield and quality are critical for success. This RIPA Buffer I solves the common challenge of incomplete extraction, ensuring that both cytoplasmic and nuclear proteins are effectively released. Its concentrated formula allows researchers to adjust the final working strength based on specific sample requirements, saving time and resources while reducing experimental variables. By choosing this reagent, you ensure consistent, clean samples that lead to sharper bands in electrophoresis and more accurate quantification data.

Usage / How to Use

To use the BC4475 RIPA Buffer I, dilute the 2X concentrate to a 1X working solution using high-purity deionized water. Add appropriate protease and phosphatase inhibitors to the working solution immediately before use to prevent sample degradation. Add the chilled 1X buffer to your cell pellet or tissue sample, incubate on ice for 15 to 30 minutes, and centrifuge at high speed to collect the supernatant containing the solubilized proteins.

Why Choose This Product

– Superior batch-to-batch consistency ensures experimental reproducibility

– Developed by scientists for demanding molecular biology applications

– Proven formula that simplifies complex laboratory protocols

– Fast shipping and reliable storage stability for long-term use.