Hygromycin B 1g – CAS 31282-04-9 Laboratory Grade

Description

Introduction

Hygromycin B is a powerful aminoglycoside antibiotic derived from Streptomyces hygroscopicus that serves as a gold-standard selection agent in molecular biology. This high-purity reagent is specifically formulated to provide reliable results in both prokaryotic and eukaryotic cell culture applications.

Key Features

– High potency for effective selection of transfected cells.

– Standardized CAS number 31282-04-9 ensures research grade quality.

– Stable formulation optimized for long-term laboratory storage.

– Highly soluble in aqueous solutions for easy media preparation.

– Convenient 1g pack size suitable for small and large-scale experiments.

Benefits

Using high-quality Hygromycin B allows researchers to successfully identify and isolate transformed cell populations with minimal background interference. Its ability to inhibit protein synthesis by interfering with ribosomal translocation makes it an indispensable tool for stable cell line generation. By choosing our product, you reduce experimental variability and ensure your gene expression studies remain on schedule.

Usage / How to Use

Hygromycin B is primarily used as a selective agent in cell culture media. It is added to growth media to kill non-transfected cells that lack the resistance gene, typically the hph gene. Researchers should perform a kill curve experiment to determine the optimal concentration for their specific cell line, as sensitivity varies depending on the species and culture conditions. Once determined, it is used to maintain stable cell lines during continuous passage.

Why Choose This Product

– Guaranteed purity levels for reproducible scientific data.

– Stringent quality control protocols for consistent batch-to-batch performance.

– Cost-effective 1g volume minimizes waste for laboratories.

– Trusted by researchers for demanding genetic selection protocols.

– Fast and reliable delivery to keep your laboratory workflow uninterrupted.