Bluo-Gal BC1182 High-Purity Molecular Biology Reagent
Experience superior screening results with Bluo-Gal BC1182. This high-purity chromogenic substrate is essential for blue-white colony screening in molecular cloning experiments. Designed for researchers demanding precision and reliability, it ensures clear color development and consistent performance in your lab workflows. Elevate your genetic research with a reagent you can trust.
Product successfully added to the Quote List
Description
Introduction
Bluo-Gal BC1182 is a premium-grade halogenated indolyl-beta-D-galactopyranoside substrate engineered for high-performance molecular biology applications. It serves as an essential tool for identifying recombinant bacteria, providing researchers with reliable and sharp colorimetric results during critical cloning and genetic screening protocols.
Key Features
– High-purity chromogenic substrate for blue-white colony selection.
– Exceptional solubility in dimethylformamide (DMF) and other common solvents.
– Provides intense, clear blue coloration for easy visual identification of recombinants.
– Stable formulation designed for consistent shelf-life and experimental repeatability.
– Optimized for use with LacZ-based cloning vectors and competent cells.
Benefits
The use of Bluo-Gal BC1182 simplifies the screening process by delivering distinct, high-contrast color markers that eliminate ambiguity. By providing a clear distinction between transformed and non-transformed colonies, this reagent saves valuable time and minimizes errors in genetic analysis. Its superior stability ensures that your results remain consistent across different batches, giving you the confidence needed for high-stakes laboratory research and repetitive cloning workflows.
Usage / How to Use
Bluo-Gal is typically used as a supplement in agar plates or top agar during microbial transformation procedures. First, dissolve the powder in an appropriate solvent such as DMF or DMSO to create a concentrated stock solution. Prepare your LB agar medium and allow it to cool to approximately 50 degrees Celsius before adding the Bluo-Gal stock along with an inducer like IPTG. Pour the plates and allow them to solidify. Once your transformed bacteria are plated, the presence of active beta-galactosidase will cleave the Bluo-Gal, resulting in a deep blue pigment within the bacterial colonies.
Why Choose This Product
Researchers choose Bluo-Gal BC1182 because it offers unmatched reliability in sensitive molecular biology assays. Our commitment to strict quality control ensures that every batch meets the highest standards for purity, preventing unwanted background noise in your screenings. With its proven track record in standard lab protocols, this product is a staple for both academic research institutions and commercial biotech firms looking for reproducible, high-quality results in every experiment.
Additional information
| brands | |
|---|---|
| capacity |
General Inquiries
There are no inquiries yet.
Reviews
There are no reviews yet.