BC0202 Fluorescein Diacetate Cell Viability Probe
Achieve accurate cell viability assessment with BC0202 Fluorescein Diacetate. This high-purity, non-fluorescent ester easily penetrates live cell membranes, where it is hydrolyzed by intracellular esterases to produce bright green fluorescence. Ideal for flow cytometry and fluorescence microscopy, it provides reliable results for your critical research and diagnostic applications.
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Description
Introduction
Fluorescein diacetate (FDA) is a premier fluorogenic substrate widely used in biological research to determine cell viability and enzymatic activity. BC0202 is designed for laboratories requiring high-purity reagents to ensure consistent and reproducible results in complex cellular staining experiments.
Key Features
– High chemical purity grade for consistent intracellular hydrolysis
– Rapid cell membrane permeability for efficient staining
– Strong green fluorescence output upon enzymatic conversion
– Compatible with standard flow cytometers and fluorescence microscopes
– Stable storage profile when kept under recommended conditions
– Reliable marker for metabolic activity in living cells
Benefits
By using the BC0202 Fluorescein Diacetate, researchers can quickly distinguish between live and dead cell populations with high precision. Because it is only converted to fluorescent fluorescein by active intracellular esterases, it serves as an excellent indicator of true metabolic vitality rather than just membrane integrity. This allows for more nuanced data collection in toxicology, drug screening, and fundamental cell biology studies, saving you time and reducing the risk of false-positive results in your experimental data.
Usage / How to Use
This product is typically used in aqueous buffer solutions. A small amount is added to a cell suspension, where it diffuses into the cytosol. Once inside, cytoplasmic esterases cleave the diacetate groups to release the fluorescent compound, which is then retained by intact cell membranes. Researchers should incubate for a short period at physiological temperatures before analyzing the samples using a flow cytometer or a fluorescence microscope equipped with standard FITC excitation and emission filters.
Why Choose This Product
Quality assurance is our top priority, ensuring that BC0202 meets the rigorous standards required for modern cell analysis. By choosing this reagent, you are investing in a proven, stable, and highly sensitive probe that minimizes background noise. Our consistent supply chain ensures you have access to this essential tool whenever your research demands it, backed by our commitment to scientific excellence and support.
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