BC4478 5x RIPA Buffer IV with Triton-X-100 pH 7.4 Concentrate
Streamline your protein extraction with our 5x RIPA Buffer IV. Formulated with high-grade Triton-X-100 at pH 7.4, this concentrate ensures efficient cell lysis and optimal protein recovery. Perfect for Western blotting, immunoprecipitation, and reporter assays, our stable formula saves time while providing the reliable, reproducible results your laboratory research demands every day.
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Description
Introduction
The BC4478 5x RIPA Buffer IV is a premium laboratory-grade lysis solution engineered for robust protein extraction. By utilizing a optimized concentration of Triton-X-100 at a precise pH of 7.4, this buffer effectively disrupts cell membranes while maintaining the integrity and activity of delicate target proteins for downstream analysis.
Key Features
– Optimized 5x concentration for flexible working dilutions
– Formulated with Triton-X-100 for superior membrane solubilization
– Precise pH 7.4 level to ensure stability of protein samples
– Highly compatible with standard Western Blotting workflows
– Designed for low-background and high-yield protein recovery
– Stable storage life for consistent performance over time
Benefits
Using this 5x RIPA Buffer IV significantly reduces the time spent on manual buffer preparation, allowing your team to focus on critical data analysis. Because the formula is highly consistent, you eliminate the risk of batch-to-batch variability. This ensures that your protein extracts are clean, concentrated, and ready for sensitive applications like immunoprecipitation, preventing sample loss and improving the reproducibility of your scientific experiments.
Usage / How to Use
This buffer is supplied as a 5x concentrate. To use, dilute the required amount with high-purity deionized water to reach a 1x working concentration. Add protease inhibitors if necessary before applying to your cell pellets. Incubate on ice for 15 to 30 minutes, centrifuge at high speeds to remove debris, and collect the supernatant. The resulting lysate is ideal for immediate use or long-term storage at -20 degrees Celsius.
Why Choose This Product
Researchers choose our RIPA buffer because of our commitment to chemical purity and rigorous quality control. We understand that your experiments rely on high-quality reagents to produce publication-ready data. By choosing BC4478, you benefit from a reliable, industry-standard formulation that simplifies your laboratory workflow, reduces waste through its concentrated format, and offers the professional consistency needed for successful biomedical research.
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